Organoids are 3D organ-like structures assembled in vitro from adult stem cells derived from primary tissues. They can differentiate into multiple organ-specific cell types and exhibit interactions and spatial morphologies between cells and surrounding matrix, reproducing some key functions and structures of real human organs in vitro. They also have stable phenotypic and genetic characteristics.
As an emerging biological model, organoids have unique advantages compared to cell lines and animal models. They have cellular composition and structure that more closely resemble human tissues, providing a highly physiologically relevant system, making them an excellent model for basic research. Moreover, organoid models are more cost-effective and have higher timeliness compared to experimental animals, thus improving research efficiency.
Advantages of organoid models include:
Tumor Organ Drug Sensitivity Testing Service
Tumor organ personalized drug sensitivity testing is a three-dimensional model established through the in vitro 3D culture system of fresh tumor tissue from patients, which is highly similar to the pathological histological features, molecular features, and drug sensitivity of the patient's tumor. Then, the efficacy of clinical treatment plans is quickly evaluated using the tumor organ model, and the most suitable treatment plan for the patient is selected, aiming to achieve precise personalized medication for the tumor patient.
Scientific Research Services
1. Construction of Organoids
2. Establishment of Disease-relevant Models and Mechanism Research
3. Drug Efficacy and Mechanism Research
Tumor Personalized Drug Sensitivity Testing
O-Avatar Tumor Organoid Drug Sensitivity Testing
It is a cellular model that is highly similar to the characteristics of tumor patients, established through in vitro 3D culture system using fresh tissues from tumor patients. It has been proven to be highly consistent with the pathological histological features, molecular features, and drug sensitivity of the tumor, and can serve as a surrogate for in vitro drug testing.
Scope of Application:
√ Patients with multidrug resistance to chemotherapy drugs and targeted drugs, to assist in formulating subsequent treatment plans
√ Tumor patients undergoing preoperative neoadjuvant therapy, postoperative treatment, or unable to undergo surgery, to assist in formulating individualized medication plans
√ Patients with resistance to first-line or second-line treatments, to screen for cross-cancer clinical drug treatment options
√ Patients with positive genetic testing, to undergo in vitro targeted validation using gene editing techniques
√ Patients with negative genetic testing, to screen for the optimal treatment plan
√ Patients with multiple gene mutations and multiple target points, to assist in formulating appropriate targeted treatment plans
√ Other patients recommended by clinical doctors for tumor drug sensitivity testing, to assist in precise treatment.
Organoid Services for Scientific Research
1. Construction of Organoids and Establishment of Biobank
Adult stem cells or induced pluripotent stem cells (iPSCs) derived from tissues are cultured in a three-dimensional environment, induced with specific growth factors in the culture medium, to form organoids with specific three-dimensional structures and functions.
2. Drug Efficacy and Mechanism Research
Based on the constructed organoids or biospecimen library, drugs are evaluated for their efficacy. Through protein and gene-level studies, the potential mechanisms for drug treatment of diseases are analyzed.
3. Organoid Gene Editing
Using CRISPR-cas9 technology, gene editing is performed on the constructed organoids to achieve knockout, knock-in, overexpression, and mutation of target genes.
